Figure 5
From: Adiponectin is an endogenous anti-fibrotic mediator and therapeutic target
The anti-fibrotic effects of ADP355 are mediated by adiponectin receptor signaling and AMP protein kinase activation. Confluent cultures of human (a,b) or mouse (c) fibroblasts were incubated in media with ADP355 for 24 h (a,c) or 60 min (b). (a) Fibroblasts were transfected with AdipoR1/R2 siRNA (siR1/2), or scrambled control siRNA for 24 h prior to ADP355 and TGF-β. Results of qPCR shown as means ± SD of triplicate determinations. *p < 0.05, **p < 0.001. (b) Fibroblasts were immunostained with antibodies to phospho-AMP kinase (Thr172) (p-AMPK, green), or stained with DAPI (blue), and examined by confocal microscopy. Representative immunofluorescence photomicrographs. Original magnification x400. (c) Total RNA was examined by real-time qPCR. Results are the mean ± SD of triplicate determinations. *p < 0.05.
