Table 3 Identification and characterization of differential expression proteins by MALDI/TOF/TOF MS between acetate or phenol as the sole carbon.

From: Acinetobacter sp. DW-1 immobilized on polyhedron hollow polypropylene balls and analysis of transcriptome and proteome of the bacterium during phenol biodegradation process

Identified proteins

Mas scorea

Spot No.b

GI No.

Calculated pI value c

Sequence coverage (%)

Mass (Da)d

Proteins location

Express ion of acetate/acetate plus phenol (fold change)

Aconitate hydratase B

169

1

1310689

4.99

22

95384

Cytoplasm

↓ 2.67829

TonB-dependent Receptor Plug domain protein

136

2

1310709

5.10

28

36470

Outer membrane

↓ 2.01854

Urocanate hydratase OS

353

3

470

5.63

26

59646

Cytoplasm

↓ 2.73441

60 kDa chaperonin, groL

193

4

903916

4.92

24

57189

Cytoplasm

↓ 1.98799

Serine hydroxymethyltransferase,

173

5

557600

5.44

31

45195

Cytoplasm

↓ 2.10096

3-oxoadipyl-CoA thiolase

270

6

1310697

5.89

51

41279

Cytoplasm

↑ 2.18868

Phenol hydroxylase P5 protein

124

7

1310911

4.53

18

39500

Cytoplasm

↑ Only in acetate + phenol

Pirin family protein OS

147

8

1310689

4.96

13

35227

Cytoplasm

↑ Only in acetate + phenol

Muconate and chloromuconate cycloisomerases family protein

196

9

1343073

5.41

27

39275

Cytoplasm

↑ Only in acetate + phenol

Catechol 1,2-dioxygenase, catA

140

10

1343073

4.70

37

33526

Cytoplasm

↑ 24.5042

MetA-pathway of phenol degradation family protein OS

81

11

1358412

5.30

26

32212

Outer membrane

↑ 9.57105

3-oxoadipate CoA-transferase subunit B (Fragment)

181

12

1310659

4.86

42

22789

Cytoplasm

↑ Only in acetate + phenol

3-oxoadipate CoA-transferase subunit A (Fragment)

149

13

1310720

7.66

46

19886

Cytoplasm

↑ Only in acetate + phenol

Uncharacterized protein

133

14

1148157

4.85

19

23098

Cytoplasm

↑ 1.81059

Nitroreductase family protein

118

15

1310689

5.83

41

21938

Cytoplasm

↑ Only in acetate + phenol

Fimbrial protein

205

16

575584

7.77

39

13897

Outer membrane

↑ Only in acetate + phenol

OsmC-like family protein

176

17

1310689

5.71

66

14449

Cytoplasm

↑ Only in acetate + phenol

Twitching mobility protein, pilT

40

18

1310605

6.27

23

35731

Cytoplasm

↑ Only in acetate + phenol

Cupin domain protein

112

19

1310689

5.37

25

26710

Cytoplasm

↑ Only in acetate + phenol

Isocitrate lyase

216

20

470

5.30

19

59646

Cytoplasm

↑ Only in acetate + phenol

  1. aMOWSE scores of the highest confident matches (P < 0.05).
  2. bThe number refers to the spot numbers as given in Fig. 6.
  3. cpI, the predicted isoelectric point calculated from the protein sequence.
  4. dThe predicted molecular weight calculated from the protein sequence.
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