Table 3 SULTs Activities and Expression differences in Pericarcinomatous, Tumor and Healthy tissues.

From: LC-MS/MS quantification of sulfotransferases is better than conventional immunogenic methods in determining human liver SULT activities: implication in precision medicine

Enzyme

No. of Quantified Donor

Probe Substrate Activities

Protein Amounts

mRNA Expression Levels

Mean

SD

Max

Min

Fold

Mean

SD

Max

Min

Fold

Mean

SD

Max

Min

Fold

Pericarcinomatous Tissues

pmol/mg/min

pmol/mg protein

relative gene expression rate

 #SULT1A1

10

63.7

50.9

155.2

12.0

12.9

13.2

10.3

33.6

2.8

12

0.9

0.2

1.0

0.6

1.6

 #SULT1A3a

10

11.6

8.4

28.6

0.6

47.7

8.2

2.3

11.2

2.8

4

1.0

0.0

1.1

1.0

1.1

 #SULT1A3b

10

2.2

1.6

5.7

0.4

14.2

8.2

2.3

11.2

2.8

4

1.0

0.0

1.1

1.0

1.1

 #SULT1B1

10

5.7

3.1

11.3

2.1

5.4

7.6

2.7

11.8

2.6

4.5

1.0

0.1

1.2

0.8

1.5

 #SULT1E1

10

113.2

89.0

269.1

12.1

22.2

1.2

0.4

1.7

0.6

2.8

1.1

0.1

1.2

1.0

1.2

 #SULT2A1

10

41.1

24.9

86.7

6.2

13.9

42.4

24.0

77.0

12.6

6.1

1.0

0.0

1.1

1.0

1.1

Tumor Tissues

 SULT1A1

10

22.9

35.4

96.8

1.1

88

6.4

7.9

21.6

0.2

108

0.4

0.5

1.7

0.0

1.7

 SULT1A3a

10

48.2

105.6

345.1

2.4

143.8

7.7

3.6

14.3

3.4

4.2

0.7

0.1

2.7

0.2

13.5

 SULT1A3b

10

10.4

22.2

72.7

0.8

90.9

7.7

3.6

14.3

3.4

4.2

0.7

0.1

2.7

0.2

13.5

 SULT1B1

10

9.8

8.9

25.4

0.8

31.8

12.2

4.9

20.9

6.4

3.3

1.0

0.4

1.6

0.6

2.7

 SULT1E1

10

126.9

149.7

466.4

4.9

95.2

1.1

0.8

2.5

0.2

12.5

0.5

0.7

2.2

0.0

2.2

 SULT2A1

10

20.4

16.6

39.4

0.4

98.5

29.9

24.2

73.1

3.4

21.5

0.2

0.3

1.1

0.0

1.1

Healthy Tissues

 SULT1A1

10

114.1

27.9

151.0

64.2

2.4

23.7

12.9

48.5

12.1

4.0

—

—

—

—

—

 SULT1A3a

10

10.2

4.0

16.8

4.4

3.8

5.2

2.0

8.2

2.2

3.7

—

—

—

—

—

 SULT1A3b

10

3.3

2.2

7.9

0.7

11.3

5.2

2.0

8.2

2.2

3.7

—

—

—

—

—

 SULT1B1

10

4.2

1.7

7.1

2.2

3.2

5.4

2.2

8.8

1.8

4.9

—

—

—

—

—

 SULT1E1

10

171.3

134.3

439.6

60.3

7.3

2.4

0.9

4.2

1.5

2.8

—

—

—

—

—

 SULT2A1

10

76.80

7.7

89.7

66.0

1.4

63.6

19.9

90.9

31.1

2.9

—

—

—

—

—

  1. aThe metabolite is dopamine 3-O-Sulfate.
  2. bThe metabolite is dopamine 4-O-Sulfate.
  3. #One concentration of probe substrate activities for each SULT: 1 μM p-nitrophenol for SULT1A1; 0.625 μM dopamine for SULT1A3; 2.5 μM 2-aminophenol for SULT1B1; 10 μM 17β-estradiol for SULT1E1 and 1 μM DHEA for SULT2A1.
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