Table 5 Summary of turnover numbers of probe substrate metabolized by SULT enzymes.

From: LC-MS/MS quantification of sulfotransferases is better than conventional immunogenic methods in determining human liver SULT activities: implication in precision medicine

Isoforms

TON (hHLS9-individual)a

TON (nHLS9-individual)b

TON (tHLS9-individual)c

nd

Mean

SD

95%CIe

Mean

SD

95%CI

Mean

SD

95%CI

pmol metabolite formed/min/pmol SULT enzymes

SULT1A1

5.4

1.5

4.4~6.5

4.1f

1.4

3.1~5.1

2.8f

1.5

1.8~4.0

10

SULT1A3g1

2.2

1.4

1.2~3.3

1.5

1.3

0.5~2.4

4.6

7.9

1.1~10.2

10

SULT1A3g2

0.7

0.4

0.4~0.9

0.3

0.2

0.1~0.5

1.0

1.7

0.2~2.2

10

SULT1B1

1.0

0.9

0.4~1.6

0.9

0.9

0.3~1.6

0.9

0.8

0.3~1.4

10

SULT1E1

106.6

100.3

34.9~178.4

100.9

88.9

37.3~164.5

132.4

140.31

32.0~232.8

10

SULT2A1

1.3

0.5

0.97~1.7

1.2

1.0

0.4~1.9

1.3

1.3

0.4~2.2

10

  1. a,b,cTurnover numbers of probe substrates metabolized by SULT enzymes in hHLS9-individual, nHLS9-individual and tHLS9-individual, which were calculated as the ration of maimal metabolic rate (Vmax) to the total amount of SULTs enzyme that is shown in Figs 4 and 6.
  2. dNumbers of donors were calculating turnover numbers.
  3. e95% CI represents the 95% confidence interval. Paired-samples t test or Wilcoxon sign rank test was used for data analysis.
  4. fStatistical significance (P < 0.05).
  5. g1,g2Dopamine was the probe substrate of the SULT1A3. Dopamine has two isomeride metabolites: dopamine 3-O-Sulfate and dopamine 4-O-Sulfate.
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