Figure 1

Bioinformatic analysis of CCLE and TCGA datasets for EMT-associated signatures. (a) Flow chart describing the generation of the shared pan-cancer CCLE/TCGA EMT-associated mRNA signature. (b) Heatmap of 201 mRNAs that showed significant differences in expression between epithelial-like and mesenchymal-like cancer cell lines within the analysed cancer entities (log2 fold change of expression is shown; p < 0.05). (c) Heatmap showing Spearman correlation coefficients of 365 epithelial and mesenchymal state-associated mRNAs with CDH1 and VIM. Epithelial state-associated mRNAs were defined as mRNAs that positively correlate with expression of the epithelial marker CDH1 (r > 0.1) and negatively correlate with expression of the mesenchymal marker VIM (r < −0.1) in all indicated TCGA datasets. Mesenchymal state-associated mRNAs were defined as mRNAs that negatively correlate with expression of the epithelial marker CDH1 (r < −0.1) and positively correlate with expression of the mesenchymal marker VIM (r > 0.1) in all indicated TCGA datasets. COAD, colon cancer; BRCA, breast cancer; LUAD, lung cancer; HNSC, head&neck squamous cel cancer; PRAD, prostate adenocarcinomas; BLCA, bladder cancer; PAAD, pancreatic adenocarcinomas.