Figure 4
From: Pan-cancer EMT-signature identifies RBM47 down-regulation during colorectal cancer progression
Low expression of RBM47 protein is associated with metastasis and positive nodal status in CRC patients (a) Association of RBM47 protein expression with liver metastasis (M1) and nodal metastasis (N+) in the M0/M1 patient collection (N = 86). Left panel: representative IHC staining results of patient samples with staining scores. Right panel: quantification of IHC staining and association with metastasis (M0 – no metastases present, M1 – liver metastases present) and nodal status (N0 – no tumor cells present in lymph nodes, N+ - tumor cells present in lymph nodes). The scale bar in low magnification images (upper part) represents 100 μm and the bar in high magnification images (lower part) represents 50 μm. (b) Expression of RBM47 mRNA in normal colonic tissue and colon tumors in the TCGA COAD dataset. In the left panel expression of RBM47 in paired samples from the same patient are shown and in the right panel all samples are shown. (c) Exemplary IHC results showing expression of RBM47 protein in a colon cancer (red arrow) and adjacent normal colonic mucosa (green arrow). The scale bar represents 100 μm. (d) Immunoblot analysis of RBM47 expression in a cell line panel consisting of epithelial-like (E) and mesenchymal-like (M) colorectal (CRC), breast (BC), and prostate (PC) cancer cell lines.
