Figure 6

Direct suppression of RBM47 by the EMT-TFs SNAIL and SLUG. (a) qPCR analysis of RBM47 mRNA and (b) Western blot analysis of the indicated proteins in DLD1 cell pools harboring pRTR/SNAIL plasmids. In (a) cells were treated with DOX or vehicle for 72 hours, in (b) with DOX for the indicated periods. (c and d) as in (a) and (b) but using DLD1 cell pools harboring pRTR/SLUG plasmids. (e) Phase contrast microscopy of the DLD-pRTR/SNAIL and DLD-pRTR/SLUG cell lines treated with vehicle or DOX for 48 hours. Scale bars represent 50 μm. (f) qChIP analysis of SNAIL occupancy at the RBM47 promoter and, as a control, the acetylcholine receptor (ACHR) locus in SW480 cells. In a, c, and f mean values ± SD (n = 3) are provided. *P < 0.05; **P < 0.01; ***P < 0.001.