Figure 7

Inhibition of RBM47 induces EMT, cell migration and invasion. (a) Phase contrast microscopy of the indicated CRC cell lines transfected with control or RBM47-specific siRNA. Scale bars represent 100 μm. (b) Western blot analysis of the indicated proteins in CRC cell lines transfected with control or RBM47-specific siRNA for 72 h. (c) qPCR analysis of the indicated mRNAs in the indicated CRC cell lines transfected with control or RBM47-specific siRNA for 72 h. (d) Wound healing assay of the indicated CRC cell lines transfected with control or RBM47-specific siRNA 48 hours before a scratch was generated. (Upper panels) Representative photographs of the initial wound area and the same area 24 hours later. (Lower panels) Quantification of wound closures: The width of scratches in 2 independent wells was analyzed for each state. Results represent the average (%) of wound closure. (e) Relative invasion of indicated CRC cells in matrigel-coated Boyden chambers transfected with control or RBM47-specific siRNA for 48 hours. In c, d, and e mean values ± SD (n = 3) are provided. *P < 0.05; **P < 0.01; *** P < 0.001.