Figure 2 | Scientific Reports

Figure 2

From: Blocking autophagy improves the anti-tumor activity of afatinib in lung adenocarcinoma with activating EGFR mutations in vitro and in vivo

Figure 2

Afatinib-induced apoptosis is partially caspase 3-dependent in H11975 and H1650 cells. (A) H1975 and H1650 cells were stimulated by indicated concentrations of afatinib for 48 h, then caspase 3 activity was detected by the caspase 3 activity kit. Data were represented as mean ± SD (*P < 0.05, **P < 0.01, ***P < 0.001 versus control) (B–D) H1975and H1650 cells were treated with 10 μM afatinib in the absence or presence of 20 μM z-VAD-fmk for 48 h. (B) Cell viability was measured by MTT assay at 570 nm. (C) The activity of caspase 3 were determined by the caspase 3 activity kit. All data were represented as mean ± SD (*P < 0.05). (D) The levels of PARP and cleaved-PARP were measured by western blot analysis. These blots were cropped with Photoshop CS6.

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