Figure 3 | Scientific Reports

Figure 3

From: Blocking autophagy improves the anti-tumor activity of afatinib in lung adenocarcinoma with activating EGFR mutations in vitro and in vivo

Figure 3

Afatinib induces autophagy in H1975 and H1650 cells. (A) H1975 and H1650 cells were treated with 10 μM afatinib for 24 h. The autophagosomes were observed using TEM (white arrows indicated the autophagosomes). (B) H1975 and H1650 cells were stimulated by 10 μM afatinib for 24 h, then stained by Cyto-ID® autophagy dye and examined using confocal fluorescent microscopy. 50 nM Rapamycin was used as positive control. (C) H1975 and H1650 cells were treated with 2.5, 5.0, 10 and 20 μM of afatinib for 24 h, then the level of LC3-I/II were measured by western blot analysis. These blots were cropped with Photoshop CS6. The concentration of LC3-I and LC3-II were quantified by ImageJ. (D) H1975 and H1650 cells were treated with 10 μM afatinib for 0, 3, 6, 12 and 24 h, the production of LC3-I/II were measured with western blot analysis. These blots were cropped with Photoshop CS6. The concentration of LC3-I and LC3-II were quantified by ImageJ.

Back to article page