Figure 4 | Scientific Reports

Figure 4

From: Blocking autophagy improves the anti-tumor activity of afatinib in lung adenocarcinoma with activating EGFR mutations in vitro and in vivo

Figure 4

Afatinib triggers autophagic flux in H1975 and H1650 cells. (A) H1975 and H1650 cells were simulated by 10 μM afatinib in the presence or absence of 20 μM CQ for 0, 12, 24 and 48 h. The production of LC3-I/II were measured by western blot analysis. These blots were cropped with Photoshop CS6. (B) H1975 and H1650 cells were treated with 10 μM of afatinib for 0, 12, 24 and 48 h and the autophagic fluxes were detected with confocal fluorescent microscopy (C) The ImageJ was used to perform the quantitation analysis in the Fig. 4B. These data were presented as mean ± SD (*P < 0.05 versus control).

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