Figure 6 | Scientific Reports

Figure 6

From: Blocking autophagy improves the anti-tumor activity of afatinib in lung adenocarcinoma with activating EGFR mutations in vitro and in vivo

Figure 6

The afatinib-induced autophagy is related to the Akt/mTOR and Erk signaling pathways. (A and B) H1975 and H1650 cells were stimulated by various concentrations of afatinib for 24 h. (A) The levels of Akt, p-Akt, mTOR, p-mTOR, p-P70S6K and p-4EBP1 were analyzed by western blot. (B) The levels of Erk 1/2 and p-Erk1/2 were analyzed by western blot. (C and D) H1975 and H1650 cells were treated with 10 μM afatinib for 0, 3, 6, 12 and 24 h. (C) The levels of Akt, p-Akt, mTOR, p-mTOR, p-P70S6K and p-4EBP1 were analyzed by western blot. (D) The levels of Erk 1/2 and p-Erk1/2 were detected by western blot. (E and F) H1975 and H1650 cells were treated with 10 μM afatinib in combination with or without 5 mM of NAC for 24 h. (E) The levels of Akt, p-Akt, mTOR, p-mTOR and p-P70S6K were analyzed by western blot. (F) The levels of Erk1/2 and p-Erk1/2 were analyzed by western blot. (A–F) All blots were cropped with Photoshop CS6.

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