Figure 2 | Scientific Reports

Figure 2

From: Molecular switching system using glycosylphosphatidylinositol to select cells highly expressing recombinant proteins

Figure 2

Generation of the PGAP2-KO cell line. (A) After the PGAP2 gene was knocked out, the expression of CD59 in a clonal cell line named PGAP2-KO3 was analysed. Compared with the wild-type HEK293 (WT), the expression level had decreased significantly in PGAP2-KO3 cells. (B) Genomic DNA was extracted from wild-type HEK293 (WT) and PGAP2-KO3 cells. The knockout region was amplified and analysed by agarose gel electrophoresis. The full image of the gel was included in the supplementary information. (C) Sequencing results revealed that there was a deletion of 66 nucleotides in PGAP2-KO3.

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