Figure 5 | Scientific Reports

Figure 5

From: Molecular switching system using glycosylphosphatidylinositol to select cells highly expressing recombinant proteins

Figure 5

Expression of LIPA as a GPI-anchored form. (A) Schematic view of the plasmid constructs of the His6-Flag (HF)-tagged secretory LIPA (sHF-LIPA) and the GPI anchored LIPA (sHF-LIPA-GPI). The signal sequence (SS) and GPI attachment signal of human CD59 were used. (B) Flow cytometric analysis of sHF-LIPA-GPI on the cell surface of P15 cells. The cells were stained with an anti-Flag antibody and a PE-conjugated goat anti-mouse IgG. After PI-PLC treatment, surface expression of sHF-LIPA-GPI was found to decrease.

Back to article page