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Figure 3

From: Candidate regulators of Early Leaf Development in Maize Perturb Hormone Signalling and Secondary Cell Wall Formation When Constitutively Expressed in Rice

Figure 3

Transgenic lines overexpressing a maize R2R3 MYB gene exhibit drooping leaves and have fewer abaxial sclerenchyma cells than wild-type. (a) T1 transgenic plants (right hand panel) are shorter than non-transgenic plants (left hand panel), and exhibit a drooping leaf phenotype. (b) T0 plant showing abaxial curling of the flag leaf (white arrow). (c) Regression plot showing vein number versus leaf width for 6 individuals from each of two independent T1 lines − 33.1 (orange spots) & 33.2 (blue spots). (d,e) Cropped gel images of genomic (d) and RT- (e) PCR reactions illustrate transgene presence and transcript levels in representatives of each T1 line. (f,g) Representative transverse leaf sections from wild-type (f) and transgenic line 33.1 (g) plants, showing presence - white arrows in (f), and absence - white arrows in (g), of abaxial sclerenchyma. Scale bar = 5 cm (a); 50 µm (f,g).

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