Figure 5 | Scientific Reports

Figure 5

From: Candidate regulators of Early Leaf Development in Maize Perturb Hormone Signalling and Secondary Cell Wall Formation When Constitutively Expressed in Rice

Figure 5

Expression of ZmWRKY12 inhibits cell wall growth in rice. (a) Non-transgenic plants (two left hand plants) are taller than plants in which expression of the transgene has been induced by germination on estradiol (two right hand plants). (b,c) Cropped gel images of genomic (b) and RT- (c) PCR reactions illustrate transgene presence in two individuals of T1 line 34.15(i) and enhanced transgene transcript levels (relative to ubiquitin) after exposure (+) of individuals from two independent T1 lines to estradiol. (d–i) Transverse cross sections of non-transgenic (d,f,h) and transgenic (e,g,i) young (d,e) and older (f–i); (h) and (i) are magnified images of (f) and (g) respectively) leaves show smaller, non-lobed mesophyll cells in transgenic plants. Scale bars = 1.5 cm (a); 75 µm (d–g); 25 µm (h,i).

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