Figure 2 | Scientific Reports

Figure 2

From: A single amino acid polymorphism in the glycosyltransferase CpsK defines four Streptococcus suis serotypes

Figure 2

Serotype switching of mutants is confirmed by dot blotting of purified CPS preparations and specific antisera. (a) The CPS from a serotype 2 field strain reacts with anti-serotype 2 but not with anti-serotype 1 sera, while the CPS from mutant strain SS2to1/2 (W161C) reacts with both antisera (top panel). The CPS from a serotype 1/2 field strain reacts with both anti-serotype 1 and anti-serotype 2 sera, while the CPS from mutant strain SS1/2to2 (C161W) reacts with anti-serotype 2 but not with anti-serotype 1 sera (bottom panel). (b) The CPS from a serotype 14 field strain reacts strongly with anti-serotype 14 and weakly with anti-serotype 1 sera, while the CPS from mutant strain SS14to1 (W161C) reacts strongly with both antisera (top panel). The CPS from a serotype 1 field strain reacts strongly with both anti-serotype 14 and anti-serotype 1 sera, while the CPS from mutant strain SS1to14 (C161W) reacts strongly with anti-serotype 14 but weakly with anti-serotype 1 sera (bottom panel).

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