Figure 2
Kinetics of BSA aggregation registered by DLS (0.1 M Na-phosphate buffer, pH 7.0, 70 °C). (A) The dependences of the light scattering intensity (I) on time obtained at the following concentrations of BSA: 1.00 (1), 1.50 (2) and 1.75 mg∙mL−1 (3). (B) The kinetic curves represented in the coordinates {(I – I 0)0.5; t} in accordance with Equation (5). The BSA concentrations are the same as on panel A. The γagg values for the right ordinate axis were calculated from Equation (6). (C) The (I − I 0)0.5 vs the portion of the aggregated protein (γagg) plot constructed at the following concentrations of BSA: 0.5 (1), 1.0 (2) and 2.0 mg∙mL−1 (3). (D) Parameter K LS as a function of \({[{\rm{BSA}}]}_{{\rm{0}}}^{{\rm{2}}}\).
