Figure 3

Adoptively transferred B cells in Apoe ^−/− /Baffr ^−/− mice produced IL10 following AngII infusion. (a–c) B cells isolated from Apoe ^−/− /Baffr ^−/− mice without or with AngII infusion for 7 days in the presence or absence of adoptively transferred CD45.1 B cells were stimulated ex vivo with anti-CD40/anti-IgM. n = 4 per group. (a) Quantitative analysis (left) and representative dot plots (right) of CD1d^hiCD5⁺ regulatory B cells gated on B220⁺ population shows a significant increase in regulatory B cell population in mice infused with AngII for 7 days. (b) Quantitative analysis (left) and representative dot plots (right) of intracellular IL10 staining in B cells gated on B220⁺ population shows a significant increase in B10 cells in mice infused with AngII for 7 days. (c) Mean fluorescence intensity of IL10 staining gated on CD19⁺ CD45.1⁻ resident B cells and CD19⁺ CD45.1⁺ transferred B cells from Apoe ^−/− /Baffr ^−/− mice adoptively transferred with CD45.1 B cells and infused without or with AngII infusion. n = 4–5 per group. (d) Quantitative analysis of Il10 mRNA expression from resident CD45.1⁻ and transferred CD45.1⁺ B cells isolated from Apoe ^−/− /Baffr ^−/− mice adoptively transferred with CD45.1 B cells and infused without or with AngII infusion. n = 4–5 per group. (e) Splenic B cells isolated from Apoe ^−/− /Baffr ^−/− mice without or with AngII infusion for 7 days in the presence or absence of adoptively transferred CD45.1 B cells were stimulated ex vivo with LPS to detect soluble IL10 levels by ELISA. n = 4 per group. *p < 0.05, **p < 0.01, ****p < 0.001.