Figure 4
From: Angiotensin II synergizes with BAFF to promote atheroprotective regulatory B cells
Regulatory B cells generated in response to AngII are atheroprotective through their production of IL-10. Apoe −/− /Baffr −/− mice were replenished with Il10 +/+ or Il10 −/− B cells and infused with AngII for 28 days. (a) Quantitative analysis (left) and representative dot plots (right) of intracellular staining of IL10 in total splenocytes demonstrates a significant reduction of IL10 levels in mice transferred with Il10 −/− B cells. n = 6 mice per group. (b) Quantitative analysis (left) and representative dot plots (right) of intracellular staining of IL10 in splenic B cells demonstrates a significant reduction of B10 in mice transferred with Il10 −/− B cells. n = 6 mice per group. (c) Quantitative lesion assessment by Oil Red O staining in the aortic sinus (left) and representative pictures (right) of Apoe −/− /Baffr −/− mice transferred with Il10 +/+ or Il10 −/− B cells and infused with AngII for 28 days (n = 6–8 mice per group). *p < 0.05. Scale bar 200 μm.
