Figure 1

The percent hemolysis of RBCs incubated with various sizes of ZnONPs at different time and various solvent conditions. (A) >100 nm ZnONPs at different concentration (25, 50, 100, 200, 400, 800, 1600 µg ml−1) dispersed in 1x PBS, double distilled water and 50 µM FA solution for 24 h. The data represent the mean ± SD (n = 3) from three experiments. #Represent significant difference at P ≤ 0.05 of PBS, water and FA solution at various concentration compared to control, *represent significant difference at P ≤ 0.05 of PBS and water compared to FA solution. (B–D) <50, 50–100 and >100 nm ZnONP dispersed in water for 2 h, 4 h, 6 h, 24 h (E–G) <50, 50–100 and >100 nm ZnONPs dispersed in FA for 2 h, 4 h, 6 h, 24 h. The data represent the mean ± SD (n = 3) from three experiments. #Represents significant difference at P ≤ 0.05 of <50, 50–100 and >100 nm ZnONPs at various concentrations compared to control at particular temperature.