Figure 2
From: Mitochondrial haplotypes influence metabolic traits across bovine inter- and intra-species cybrids
OCR and ECAR assays for bovine primary cells and cybrid cells. a and c illustrating OCR and ECAR assays of primary cell lines (Y and C1). b and d illustrating OCR and ECAR assays of cybrid cells (C0+C0, C0+C1, C0+Y), C0 (MAC-T) was used as a control for C0+C0. For OCR assays, all cells exposed sequentially to oligomycin, FCCP and rotenone plus antimycin A. Non-mitochondrial respiration was subtracted from the other values, basal respiration, ATP turnover, proton leak, and spare respiratory capacity. OCR profiles were expressed as pmole O2/min/1.8 × 104 cells. The basal glycolysis rate for each cell line was estimated by determining its ECAR in the presence of glucose. The glycolytic reserve for each cell line was estimated by determining the ECAR in the presence of oligomycin. ECAR profiles were expressed as mpH/min/1.8 × 104 cells. In cybrid cells, the acronym C0 denotes a common nucleus of MAC-T, and +C0, +Y and +C1 represent the source of mitochondria. Bars indicate the standard deviation (SD), n = 6 per group. *P < 0.05, **P < 0.01 and ns P > 0.05.
