Figure 4
From: Human endothelial cells secrete neurotropic factors to direct axonal growth of peripheral nerves
Quantification of HUVEC secretion of BDNF. HUVECs were seeded at high (10,000 cell/cm2) or low (1,000 cell/cm2) densities and cultured in parallel with DRG-HUVEC co-cultures and assayed for BDNF using ELISA kits, and normalized to their respective seeding densities. Both concentrations of HUVECs secreted measurable amounts of BDNF significantly higher than EGM-2 and low density seeding of HUVECs produced higher concentrations of BDNF per cell than high density seeding of HUVECs. Data are presented as mean ± standard error. ***(p < 0.001) and brackets indicate significance from other treatments as determined by one-way ANOVA with Holm-Sidak post hoc analysis (Well sample size for each group shown in bars from 2 independent replicates).
