Figure 5
From: Human endothelial cells secrete neurotropic factors to direct axonal growth of peripheral nerves
Neutralization of BDNF reduced DRG axonal growth in HUVEC-conditioned medium. DRG explants were cultured on PDL coated wells in (A) HUVEC-conditioned medium, (B) HUVEC-conditioned medium supplemented with 125 ng/mL of BDNF neutralizing antibody, and (C) non-conditioned EGM-2 medium (control). HUVEC-conditioned medium increased the amount of axonal branching from DRG explants. Scale = 200 µm. (D) Quantification of axonal growth between HUVEC-conditioned medium, neutralized conditioned medium, and control. Neutralization of BDNF negated the significant increase of axonal growth observed from conditioned medium. Data are presented as mean ± standard error. ***(p < 0.001) and brackets indicate significance from other treatments as determined by one-way ANOVA with Holm-Sidak post hoc analysis (DRG sample size for each group shown in bars from 3 independent replicates).
