Figure 4

The structural insights of putative auxin binding site. (A) Close-up view of ligand binding pocket showing Fobs-Fcalc electron density map contoured at 2.5σ. (B) Auxin molecule show in two different orientation (shown as sticks) fitted in the F obs-F calc electron density map. (C) Intermolecular hydrogen bonds between the auxin molecule and binding site residues formed by chains B, C, E and F. WTG hexamer is shown in cartoon representation with chain B (green), chain C (cyan), chain E (red), and chain F (orange). Auxin molecule and side chains of residues are shown as sticks. The water molecules are represented as spheres. The hydrogen bonds are shown as dashed black lines.