Figure 6

Roles of seven embryonically up-regulated proteins in cell division timing during C. briggsae embryogenesis. (A) Hierarchical clustering of cell division timings of RNAi embryos with genotypes indicated on the right. Up-regulation in division paces is colored in red and black and down-regulation in division paces in green. Cells absent in an RNAi embryo were shown in grey. (B–E) Lineage trees of a wildtype and RNAi embryos rooted with cell “ABal” up to roughly 350-cell stage with horizontal line indicating a cell division and vertical line division timing. For a full lineage tree rooted with a zygote, see Fig. S1. Significant changes in cell division timing relative to wildtype (N2) are indicated by a red arrow. The genotypes are shown above the tree. (F–K) Epifluorescence, Nomarski and their superimposed micrographs for a wildtype (F–H), approximately 350-cell stage) and a let-418 RNAi embryo (arrested at approximately 200-cell stage), respectively. Note the reduced expression intensity of histone marker (HIS-72::mCherry) before (F) and after (I) the RNAi.