Table 2 Yeast two-hybrid mutagenic screens for CEH-14 and LIM-7 interactions.

pGBT9	Sequence	pGAD10
Construct	Sequence	pGAD10
LIM-7_LID		CEH-14 _LIM1+2
WT LIM-7_LID	GIGPLMVQPATPHIDNTLGGPIDIQHFAQW	+++/+++
P350A	GIGALMVQPATPHIDNTLGGPIDIQHFAQW	+++/+++
L351A	GIGPAMVQPATPHIDNTLGGPIDIQHFAQW	+++/+++
M352A	GIGPLAVQPATPHIDNTLGGPIDIQHFAQW	+++/+++
V353A	GIGPLMAQPATPHIDNTLGGPIDIQHFAQW	+++/+++
P350A/L351A	GIGAAMVQPATPHIDNTLGGPIDIQHFAQW	+++/++
L351A/M352A	GIGPAAVQPATPHIDNTLGGPIDIQHFAQW	+++/+++
G347A/I348A/G349A	AAAPLMVQPATPHIDNTLGGPIDIQHFAQW	+++/+++
P350A/L351A/M352A	GIGAAAVQPATPHIDNTLGGPIDIQHFAQW	+++/−
V353A/Q354A/P355A	GIGPLMAAAATPHIDNTLGGPIDIQHFAQW	+++/+++
A356G/T357A/P358A	GIGPLMVQPGAAHIDNTLGGPIDIQHFAQW	+++/+++
H359A/I360A/D361A	GIGPLMVQPATPAAANTLGGPIDIQHFAQW	−/−
N362A/T363A/L364A	GIGPLMVQPATPHIDAAAGGPIDIQHFAQW	+++/+++
G365A/G366A/P367A	GIGPLMVQPATPHIDNTLAAAIDIQHFAQW	+++/+++
I368A/D369A/I370A	GIGPLMVQPATPHIDNTLGGPAAAQHFAQW	+++/+++
Q371A/H372A/F373A	GIGPLMVQPATPHIDNTLGGPIDIAAAAQW	+++/+++
A374G/Q375A/W376A	GIGPLMVQPATPHIDNTLGGPIDIQHFGAA	+++/−
H359A	GIGPLMVQPATPAIDNTLGGPIDIQHFAQW	+++/+++
I360A	GIGPLMVQPATPHADNTLGGPIDIQHFAQW	+++/+++
D361A	GIGPLMVQPATPHIANTLGGPIDIQHFAQW	+++/+++
P350A/L351A/M352A/H359A	GIGAAAVQPATPAIDNTLGGPIDIQHFAQW	−/−
P350A/L351A/M352A/I360A	GIGAAAVQPATPHADNTLGGPIDIQHFAQW	−/−
P350A/L351A/M352A/D361A	GIGAAAVQPATPHIANTLGGPIDIQHFAQW	−/−

Y2H assay summary for alanine mutagenic screening of LIM-7_LID against CEH-14_LIM1+2. Results are reported as yeast growth on moderate (SD^{-H-L-W+1mM 3-AT})/strong (SD^-H-L-W-A) selective media. Yeast growth is represented as either ‘+++’, ‘++’ or ‘+’ for robust growth at all three dilution points (10°, 10⁻¹, 10⁻²), two dilution points (10°, 10⁻¹) or only on the first dilution point (10⁰), respectively. ‘−’ represents no detectable yeast growth. LIM-7_LID and CEH-14_LIM1+2 constructs were in the vectors pGBT9 and pGAD10, respectively.

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