Figure 3
From: Sorghum Dw2 Encodes a Protein Kinase Regulator of Stem Internode Length
Fine mapping of Dw2 in the BTx642 x Tx7000 RIL population. (a) QTL map of total plant height (2009) with Dw2 labeled. Plant height was measured as the length of the plant from the base of the stem at ground level to the top of the panicle. Genetic map construction and QTL analysis were performed in R/qtl using IM. The x-axis is the markers along the chromosomes and the y-axis is the LOD value. (b) Diagram of fine mapping in BTx642 x Tx7000. The diagram shows the location of the recombination breakpoints in the 2-LOD region in the eight fine mapping lines (numbers at bottom), two of these lines had more than one recombination breakpoint in the region. The markers found through DG using NgoMIV are labeled as “Ngo_”. The markers found with Sanger sequencing are labeled with “SNP_ _” with the last five digits of the gene name. The red, dashed-line box shows the refined region of Dw2. For both (b) and (c), asterisk indicates the approximate location of Dw2. (c) Diagram of the haplotypes of the four fine mapping lines with breakpoints closest to the refined region. The region between Ngo1 and Ngo3 is shown. Blue indicates that the RIL has the BTx642 allele, red is the Tx7000 allele, and grey is the region where the breakpoint is located. Dashed lines flank the refined region of Dw2. (d) The length of the first internode below the peduncle in the same lines shown in (c). Blue indicates that the line is dw2 while red is Dw2. Average (n = 4) and standard deviation is shown.
