Figure 2
From: Attenuation of Innate Immunity by Andrographolide Derivatives Through NF-κB Signaling Pathway
Andrographolide derivatives inhibited proinflammatory cytokine transcription. THP-1 cells differentiated with 10−8 M PMA were treated with 1 μg/ml LPS in the presence or absence of 3b and 5a for 6 h. The mRNA levels of (A) IL-6 and (B) IL-18 were determined by real-time PCR analysis. THP-1 cells were transfected with 50 ng/μl poly I:C using PEI in the presence or absence of 3b and 5a for 9 h. The mRNA levels of (C) IL-6 and (D) IL-18 were determined by real-time PCR analysis. (E) THP-1 cells were treated with 5 μg/ml LPS in the presence or absence of 10 μM 3a, 5b, and 6b for 6 h, or (F) THP-1 cells were transfected with 50 ng/μl poly I:C in the presence or absence of 10 μM 3a, 5b, and 6b for 9 h, the mRNA level of IL-6 was determined by real-time PCR analysis. Results are presented as mean ± SD from at least three independent experiments (**p < 0.05; n.s., non-specific; −, DMSO control).
