Figure 2 | Scientific Reports

Figure 2

From: Basal hypersecretion of glucagon and insulin from palmitate-exposed human islets depends on FFAR1 but not decreased somatostatin secretion

Figure 2

Effect of decreasing FFAR1 expression on accumulated hormone secretion from human islets cultured at 5.6 mM glucose in the absence (white bars) or presence of 0.5 mM palmitate (black bars) for 1 day. Relative FFAR1 mRNA levels (panel A) and total FFAR1 protein (panel B) at the end of culture as compared to non-mammalian target shRNA (neg) or shRNA targeting Ffar1 (Ffar1) treatment. Accumulated secretion of glucagon (panel C), insulin (panel D) and somatostatin (panel E) is shown. Results show mean ± SEM and are from n = 3–5 donor experiments. *P < 0.05 vs. control-neg, #p < 0.05 vs. palmitate-neg.

Back to article page