Figure 6

Quantification of Spiroplasma titre as Spiroplasma dnaA gene copy number normalized to the tsetse β-tubulin gene. (a) Gff whole insects from healthy/live males and prematurely dead males from the mass-rearing facility in Ethiopia (n = 6), (b) Gff whole insects from healthy/live females and prematurely dead females from the mass-rearing facility in Ethiopia (n = 9), p < 0.05. (c) Gff whole insects from healthy/live females carrying a larvae and prematurely dead females carrying a larva from the mass-rearing facility in Ethiopia (n = 6), p < 0.05. (ANOVA test was performed; statistical significant differences are indicated with an asterisk *).