Figure 1 | Scientific Reports

Figure 1

From: Homer binds to Orai1 and TRPC channels in the neointima and regulates vascular smooth muscle cell migration and proliferation

Figure 1

Increased protein expression and interaction of TRPC channels and Orai1 with STIM1 in neointima from balloon-injured rat carotid artery. H&E-stained sections of (a) sham-operated (intact) and (b) balloon-injured arteries 14 days after injury. Prominent neointima is present and narrows the lumen. Photos are taken at 10× and 40× magnification. M = media; NI = neointima; L = lumen. Protein expression levels of TRPC1, TRPC3, TRPC4, TRPC6, Orai1, and STIM1 (c–h) are upregulated in injured (media + neointima) compared to uninjured VSMCs. Co-immunoprecipitation assay using anti-STIM1 antibody to immunoprecipitate STIM1 shows increased interaction of STIM1 with (c) TRPC1; (d) TRPC3; (e) TRPC4; (f) TRPC6; and (g) Orai1 more strongly in injured VSMCs.

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