Figure 1
From: HDAC6: A Novel Histone Deacetylase Implicated in Pulmonary Arterial Hypertension
HDAC6 is overexpressed in lungs and distal pulmonary arteries (PAs) from PAH patients and animal models. (A) Representative Western blots and corresponding densitometric analyses of HDAC6 expression in lung tissues, distal PAs and isolated PA smooth muscle cells (PASMCs) from control (n = 4–8) and PAH patients (n = 4–12). (B) Double immunofluorescence staining for αSMA (green) and HDAC6 (red) and DAPI nuclear staining in lungs from control donors (n = 5) and PAH patients (n = 5), confirming the overexpression of HDAC6 in remodeled PAs. (C) Representative Western blots and corresponding densitometric analyses of HDAC6 expression in distal PAs of non-treated rats (n = 5 for each model) as well as rats exposed to Sugen-hypoxia (Su/Hx, n = 6) or monocrotaline (MCT, n = 5). (D) Double immunofluorescence staining for αSMA (green) and HDAC6 (red) and DAPI nuclear staining showing increased expression of HDAC6 in remodeled distal PAs after Su/Hx exposure or MCT injury compared to non-treated rats (n = 5 per group). Protein expression was normalized by Amido black (AB). Scale bar = 25 μm *P < 0.05 and ***P < 0.001.
