Table 1 List of antibodies used.
Antibody (clone), Host species | Antibody concentration | Application | Antibody source [Ref.] |
---|---|---|---|
CD31 (WM59), mouse | 15 µg/ml | Flow Cytometry | BD |
CD34 (563), mouse | 50 µg/ml | Flow Cytometry | BD |
CD44 (G44-26), mouse | 25 µg/ml | Flow Cytometry | BD |
CD45 (HI30), mouse | 50 µg/ml | Flow Cytometry | BD |
CD73 (AD2), mouse | 50 µg/ml | Flow Cytometry | BD |
CD90 (5E10), mouse | 100 µg/ml | Flow Cytometry | BD |
CD105 (266), mouse | 50 µg/ml | Flow Cytometry | BD |
Mouse IgG1k | 100 µg/ml | Flow Cytometry | BD |
CD49c (Integrin α3) APC (P1B5), mouse | 1.25 µg/ml | Flow Cytometry | eBioscience |
CD49f (Integrin α6) APC (GoH3), rat | 1.25 µg/ml | Flow Cytometry | eBioscience |
CD29 (Integrin β1) FITC (TS2/16), mouse | 2.5 µg/ml | Flow Cytometry | eBioscience |
CD104 (Integrin β4) eFluor 660 (439-9B), rat | 10 µg/ml | Flow Cytometry | eBioscience |
IgG1 isotype FITC (IS5-21F5), mouse | 2.5 µg/ml | Flow Cytometry | Miltenyi Biotec |
IgG1 isotype APC (IS5-21F5), mouse | 1.25–2.5 µg/ml | Flow Cytometry | Miltenyi Biotec |
IgG2b isotype APC (141945), rat | 1.25–10 µg/ml | Flow Cytometry | R&D Systems |
Integrin α3 (ASC-1), mouse | 20 µg/ml | Blocking | Millipore |
Integrin α6 (NKI-GoH3), rat | 20 µg/ml | Blocking | Millipore |
Integrin β1 (P5D2), mouse | 2.5 µg/ml | Blocking | R&D Systems |
Cytokeratin 3 (AE5), mouse | 1:100 | Immunohistochemistry | Chemicon/Millipore |
Cytokeratin 15 (LHK15), mouse | 1:500 | Immunohistochemistry | Abcam |
Integrin α3 (P1B5), mouse | 1:200 | Immunohistochemistry | Dako |
Integrin α6 (GoH3), rat | 1:100 | Immunohistochemistry | Chemicon/Millipore |
Integrin β1 (HB1.1), mouse | 1:500 | Immunohistochemistry | Chemicon/Millipore |
Ki-67 (SP6), rabbit | 1:1000 | Immunohistochemistry | Abcam |
Laminin α1 (317), rabbit | 1:500 | Immunohistochemistry | L. Sorokin63 |
Laminin α2 (401), rabbit | 1:500 | Immunohistochemistry | L. Sorokin63 |
Laminin α3, rabbit | 1:6000 | Immunohistochemistry | R. Timpl/T. Sasaki |
Laminin α4 (377), rabbit | 1:2000 | Immunohistochemistry | L. Sorokin63 |
Laminin α5 (405), rabbit | 1:4000 | Immunohistochemistry | L. Sorokin63 |
Laminin β1 (IIID9), mouse | undiluted | Immunohistochemistry | L. Sorokin63 |
Laminin β2 (409), rabbit | 1:200 | Immunohistochemistry | L. Sorokin63 |
Laminin β3, rabbit | 1:6000 | Immunohistochemistry | R. Timpl/T. Sasaki |
Laminin γ1 (3E10), rat | undiluted | Immunohistochemistry | L. Sorokin63 |
Laminin γ2 (LE4-6), rabbit | 1:4000 | Immunohistochemistry | R. Timpl/T. Sasaki |
Laminin γ3, rabbit | 1:2000 | Immunohistochemistry | R. Timpl/T. Sasaki |
N-Cadherin (6G11), mouse | 1:25 | Immunohistochemistry | Dako |
p63α, rabbit | 1:100 | Immunohistochemistry | Cell Signaling |