Table 1 List of antibodies used.

From: Laminin-511 and -521-based matrices for efficient ex vivo-expansion of human limbal epithelial progenitor cells

Antibody (clone), Host species

Antibody concentration

Application

Antibody source [Ref.]

CD31 (WM59), mouse

15 µg/ml

Flow Cytometry

BD

CD34 (563), mouse

50 µg/ml

Flow Cytometry

BD

CD44 (G44-26), mouse

25 µg/ml

Flow Cytometry

BD

CD45 (HI30), mouse

50 µg/ml

Flow Cytometry

BD

CD73 (AD2), mouse

50 µg/ml

Flow Cytometry

BD

CD90 (5E10), mouse

100 µg/ml

Flow Cytometry

BD

CD105 (266), mouse

50 µg/ml

Flow Cytometry

BD

Mouse IgG1k

100 µg/ml

Flow Cytometry

BD

CD49c (Integrin α3) APC (P1B5), mouse

1.25 µg/ml

Flow Cytometry

eBioscience

CD49f (Integrin α6) APC (GoH3), rat

1.25 µg/ml

Flow Cytometry

eBioscience

CD29 (Integrin β1) FITC (TS2/16), mouse

2.5 µg/ml

Flow Cytometry

eBioscience

CD104 (Integrin β4) eFluor 660 (439-9B), rat

10 µg/ml

Flow Cytometry

eBioscience

IgG1 isotype FITC (IS5-21F5), mouse

2.5 µg/ml

Flow Cytometry

Miltenyi Biotec

IgG1 isotype APC (IS5-21F5), mouse

1.25–2.5 µg/ml

Flow Cytometry

Miltenyi Biotec

IgG2b isotype APC (141945), rat

1.25–10 µg/ml

Flow Cytometry

R&D Systems

Integrin α3 (ASC-1), mouse

20 µg/ml

Blocking

Millipore

Integrin α6 (NKI-GoH3), rat

20 µg/ml

Blocking

Millipore

Integrin β1 (P5D2), mouse

2.5 µg/ml

Blocking

R&D Systems

Cytokeratin 3 (AE5), mouse

1:100

Immunohistochemistry

Chemicon/Millipore

Cytokeratin 15 (LHK15), mouse

1:500

Immunohistochemistry

Abcam

Integrin α3 (P1B5), mouse

1:200

Immunohistochemistry

Dako

Integrin α6 (GoH3), rat

1:100

Immunohistochemistry

Chemicon/Millipore

Integrin β1 (HB1.1), mouse

1:500

Immunohistochemistry

Chemicon/Millipore

Ki-67 (SP6), rabbit

1:1000

Immunohistochemistry

Abcam

Laminin α1 (317), rabbit

1:500

Immunohistochemistry

L. Sorokin63

Laminin α2 (401), rabbit

1:500

Immunohistochemistry

L. Sorokin63

Laminin α3, rabbit

1:6000

Immunohistochemistry

R. Timpl/T. Sasaki

Laminin α4 (377), rabbit

1:2000

Immunohistochemistry

L. Sorokin63

Laminin α5 (405), rabbit

1:4000

Immunohistochemistry

L. Sorokin63

Laminin β1 (IIID9), mouse

undiluted

Immunohistochemistry

L. Sorokin63

Laminin β2 (409), rabbit

1:200

Immunohistochemistry

L. Sorokin63

Laminin β3, rabbit

1:6000

Immunohistochemistry

R. Timpl/T. Sasaki

Laminin γ1 (3E10), rat

undiluted

Immunohistochemistry

L. Sorokin63

Laminin γ2 (LE4-6), rabbit

1:4000

Immunohistochemistry

R. Timpl/T. Sasaki

Laminin γ3, rabbit

1:2000

Immunohistochemistry

R. Timpl/T. Sasaki

N-Cadherin (6G11), mouse

1:25

Immunohistochemistry

Dako

p63α, rabbit

1:100

Immunohistochemistry

Cell Signaling