Table 2 Primers used in qRT-PCR primer assays.
Gene Symbol | Accession No. | Product Length | Probe No./SYBR | Sequence 5′–3′ |
|---|---|---|---|---|
CEACAM1 | NM_001024912.2 | 113 | P79 | TCTTTGGGGCTAAGAGAAAGG |
GAGGTACCTGAGTATAGAGAACTCCAA | ||||
GAPDH | NM_002046.3 | 72 | P3 | CAGCAAGAGCACAAGAGGAA |
GTGGTGGGGGACTGAGTGT | ||||
ICAM1 | NM_000201.2 | 73 | P30 | TTGACCTTTTGGGCTCAAGT |
TGGTGTTGTGAGCCTATGGT | ||||
Ki-67 | NM_002417.4 | 78 | P50 | TTACAAGACTCGGTCCCTGAA |
TTGCTGTTCTGCCTCAGTCTT | ||||
KIT | NM_000222.2 | 61 | P6 | GAGTAGCTTACCAGAAGCTTCCATAG |
CATAGGGACTGATGCCTTCC | ||||
KRT3 | NM_057088.2 | 92 | P74 | CCTGTGATTGTCCAGGTGTG |
ATACATCAGAGCTGTAGTGAGCATC | ||||
KRT15 | NM_002275.3 | 60 | P10 | CATTGGCATCAGGGAAGC |
TTGATGTGGAAATTGCTGCT | ||||
LAMA1 | NM_005559.3 | 149 | SYBR | CTGGGACCGAGTCCTGAAC |
ACACAACATCTCTCCCCAGAA | ||||
LAMA1 | NM_005559.3 | 60 | P38 | AGGATGACCTCCATTCTGACTT |
CCTTACATGGGCACTGACCT | ||||
LAMA2 | NM_000426.3 | 68 | P81 | GCAAGCCACTGGAGGTTAAT |
TGGGCATGATACAGGTTGAA | ||||
LAMA3 | NM_198129.1 | 72 | SYBR | GCACTGATTACCCAATGCACC |
ACCTGTCTGGTTTGTGTCCTG | ||||
LAMA3 | NM_198129.1 | 65 | P29 | CCAGGAATATGGGTTGCTTG |
GGGAGCAGCACCAGGTAAT | ||||
LAMA4 | NM_001105209.1 | 68 | P55 | GCCACACTCGTCCTTCTCTC |
CCCAGGTGAAACTCTCAAGG | ||||
LAMA5 | NM_005560.3 | 75 | P44 | ATGACTCGCTCTGTGGAGGT |
GGGGTTGGCTGTGTCCTA | ||||
LAMB1 | NM_002291.2 | 72 | P82 | AAGCCAGAAAGTTGCTGTGTATAG |
CCTTGTTCACCTCAGCCATT | ||||
LAMB2 | NM_002292.3 | 100 | P43 | AAGGCCTACCCCAGTTCCTA |
GGGTTCACACTGGTTTATTGG | ||||
LAMB3 | NM_001127641.1 | 72 | P23 | GGCATGCCATTGAAACTAAGA |
AGAACTAAAGGCGGGGGATA | ||||
LAMB4 | NM_007356.2 | 67 | P5 | CCCCACACCCTGTCCTTATT |
ATTTTCCTGGTGGCATTTCA | ||||
LAMC1 | NM_002293.3 | 92 | P66 | GCCATTATTTTATTGTCTAGCTCCA |
ATCCCTGTGTCAACCAGCAT | ||||
LAMC2 | NM_005562.2 | 96 | P65 | CACTCTGTGCCTTTCTACAACTG |
CCAAGGTGGAAGTGCCTCT | ||||
LAMC3 | NM_006059.3 | 67 | P14 | CAGGACTCCTCAGCATTTCC |
TTGCCATCTGCTGGAAGAG | ||||
SOX2 | NM_003106.3 | 85 | P65 | GGGGGAATGGACCTTGTATAG |
GCAAAGCTCCTACCGTACCA |
