Figure 3
From: Generation of novel patient-derived CIC- DUX4 sarcoma xenografts and cell lines
Characteristics of CDS cells. (a) Phase-contrast images of CDS cells cultured in conventional culture conditions and (b) in the ultra-low attachment culture dishes. Bar: 500 μm. (c,d) The growth curves for NCC-CDS1-X1-C1 (c) and X3-C1 (d) cells. (e,f) Immunohistochemical analysis for WT1 (e) and ETV4 (f). (g) CIC break-apart FISH of CDS cells. The arrows indicate the splits. (h) Sanger sequence analysis of the transcript showing CIC–DUX4 fusion (upper, NCC-CDS1-X1-C1; lower, NCC-CDS1-X3-C1). An antiparallel 4767–4774 region of CIC exon insertion is indicated in the orange box.
