Figure 2
(A) The SAXS I(Q) profiles of gelsolin (8 mg/ml) at different temperatures in buffer lacking any free Ca2+ and pH 8 are plotted here. (B) This panel shows the Kratky plots of the SAXS datasets shown in panel (A). The smoothened versions were generated by averaging adjacent 5 data points). (C) The Guinier analysis of all the datasets presuming globular scattering profiles are shown here. (D) Variation in the molecular mass of the gelsolin molecules estimated using I0 values from Guinier analysis as a function of temperature are plotted here. The blue line represents the Sigmoidal fit to the data presuming two-state transition. Inset shows the image of the native PAGE of the heated samples. Red circles indicate samples where slight aggregation was visible and thus indicate uncertainty in estimation. (E) Variation in the RG values as a function of temperature for gelsolin solution is plotted here (black squares). The blue line fitted through symbols shows the Sigmoidal fit to the observed trend in the increase of RG values. The point of half-change for RG values in samples varying in gelsolin concentration are shown in inset. (F) Variation in the calculated RG values of the different conformations present in the ensemble as a function of temperature of experiment are presented against the pool of random conformations considered.
