Figure 4
(A) The P(r) profiles computed using SAXS data profiles collected for 8 mg/ml gelsolin shown in Fig. 2A are plotted here for temperatures 10, 30 and 40 °C. (B–D) The SAXS data based dummy residue model solved for gelsolin protein at temperatures 10, 30 and 40 °C are presented. The dummy residues are shown as grey mesh. As discussed in main text, for each SAXS data based model, either crystal structure of Ca2+-free gelsolin or its simplistic reoriented model was used to assess the shape rearrangements seen in the SAXS data based dummy atom models. In all structural models used with SAXS data based models, the G1 domain and g1–g2 linker, and the C-tail latch are depicted in red and magenta coloured ribbons, respectively. Additionally, the black circle drawn highlights the location of G1 domain and the arrows between different panels track its repositioning as a function of temperature.
