Figure 6
(A) Variation in RG values of gelsolin molecules in absence of PIP2 as tracked by Guinier analyses of the SAXS datasets obtained at different temperatures are shown. The data here shows whether the RG values decrease upon lowering the sample temperature or not. (B) Computed RG values from SAXS datasets obtained from samples of gelsolin mixed with PIP2 are presented here as function of temperature. The blue arrow highlights the PIP2 addition induced decrement in RG values in gelsolin samples pre-heated to 45 °C. (C) SAXS data based scattering shape solved for gelsolin molecules at 40 and 45 °C but without and with PIP2 in solution, respectively are shown here (dummy residues shown as brown mesh). As detailed in discussion section, models were placed inside SAXS data based shape profiles for comparison. (D) The panels show the variation in RG values of gelsolin value as a function of free Ca2+ ions (left) or low pH in buffer (black open squares). The filled red squares show the RG values of the samples having same amount of Ca2+ ion or pH in buffer, plus PIP2. The black arrows highlight decrement in the RG values upon addition of PIP2.
