Table 1 Estimated molecular mass values of the scattering species in solution of gelsolin at different temperatures are tabulated below.
Temperature (°C) | Estimated I0 value (au) | Error in I0 | Calculated Mass of scattering Species (kDa) | Equivalent Error in estimation (kDa) | Comments on sample |
|---|---|---|---|---|---|
10 | 28559 | 552 | (83) | — | |
20 | 25890 | 362 | 75.2 | 1.1 | Monomer |
25 | 25602 | 259 | 74.4 | 0.8 | Monomer |
30 | 26444 | 579 | 76.9 | 1.7 | Monomer |
35 | 26686 | 387 | 77.6 | 1.1 | Monomer |
40 | 27847 | 385 | 80.9 | 1.1 | Monomer |
45 | 33202 | 561 | 96.5 | 1.6 | Association |
50 | 47278 | 785 | 137.4 | 2.3 | Association |
60 | 127729 | 3156 | 371.2 | 9.2 | Aggregation |
70 | 337280 | 2870 | 980.2 | 8.3 | Aggregation |
80 | 378500 | 3121 | 1100.0 | 9.1 | Aggregation |
