Figure 5
CMA-mediated inhibition of macroautophagy is ATG5 dependent. (A) qRT-PCR analysis of the ATG5-dependent macroautophagy gene expression levels in shLAMP2A or shControl MDA-MB-231 cells (left) (n = 3; Student’s t test, **P < 0.01, statistical comparison between the two marked treatment groups). (B) shLAMP2A or shControl MDA-MB-231 cells were starved and harvested at the indicated timepoints for immunoblotting of ATG5 and truncated ATG5. The numbers underneath each lane represent the densitometric quantification of the detected protein after normalizing to β-actin. (C) LAMP2A, ATG5 and LC3 levels in the subcutaneous tumor tissues of shLAMP2A or shControl MDA-MB-231-implanted mice were measured by immunohistochemistry.
