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Figure 1

From: Exosomes from metastatic cancer cells transfer amoeboid phenotype to non-metastatic cells and increase endothelial permeability: their emerging role in tumor heterogeneity

Figure 1

SW480 and SW620 cell-derived exosomes characterization. (A) Equal amount (15 µg) of SW480Exo and SW620Exo proteins were probed with the indicated antibodies that detect exosome-enriched proteins. Original uncropped WBs are reported in Figure S7A. (B) 30 µg of both SW480Exos and SW620Exos and cellular lysates were incubated with anti-calnexin to exclude cellular contamination in exosome preparation. Original uncropped WBs are reported in Figure S7B. WCL: Whole Cell Lysate; EXOs: exosomes. (C) Dynamic light scattering (DLS) analysis of SW480Exos and SW620Exos. Results were plotted as a % mass distribution in order to accurately represent the size distribution of the biological sample.

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