Figure 3

Immunoprecipitation/western blot analyses of hepatic AMPK-α1, nSREBP-1c and ACC-1 protein expression. Immunoblots were quantified using densitometry and normalized to GAPDH protein expression. Results are expressed as fold change compared to the AGA or AGA + NS of same age. Hepatic AMPK-α1 protein expression before (A) and after (D) GH or NS treatment. Hepatic nSREBP-1c protein expression before (B) and after (E) GH or NS treatment. Hepatic ACC-1 protein expression before (C) and after (F) GH or NS treatment. Statistical differences between the groups are given as follows: *p < 0.05: AGA vs SGA; ^# p < 0.05: AGA + NS vs SGA + NS; ^& p < 0.05: SGA + NS vs SGA + rhGH. n = 8 per experimental group.