Figure 4
Metformin increases mitochondrial biogenesis in cancer cells and inhibits mitochondrial permeability transition pore. (A) Mitochondrial DNA content and quantification of MitoTracker Green in LNCaP cells treated with metformin for 24 h. The results were obtained from three independent experiments. p < 0.05 (*); p < 0.001 (***). (B) Immunoblotting analyses of proteins and transcription factors implicated in mitochondrial function, after treatment with 5 mM metformin for 24 and 48 h. (C) Calcium retention capacity measured after the incubation of LNCaP cells with 1 µM cyclosporin A for 4 h or 5 mM metformin for 4 h and 24 h.
