Figure 3
The effects of CCM111 on the STAT3 signaling transduction pathway in HEK293 cells. (a–d) Total cell lysates were prepared for Western blot analysis to detect the protein levels. (a) The cells were treated with h-IL-6 (10 ng/ml) and CCM111 (0, 60, 120 or 160 μg/ml) for 2 hours. (b) The cells were treated with h-IL-6 (10 ng/ml) alone or in combination with CCM111 (160 μg/ml) for 1, 2 and 4 hours. (c) The cells were treated with TNF-α (10 ng/ml) and CCM111 (0, 80, 160 or 240 μg/ml) for 2 hours. (d) The cells were treated with TNF-α (10 ng/ml) alone and/or in combination with CCM111 (240 μg/ml) for 1, 2 and 4 hours. (a,b) The protein levels of STAT3 and phospho-STAT3 were detected. (c,d) The protein levels of IκBα and phospho-IκBα were detected. GAPDH served as an internal control. The full length blots images are presented in Supplementary Fig. 2. Results were obtained from three independent replicates.
