Figure 4
The effects of CCM111 on TLR signaling pathways. Three stable cell lines separately expressing TLR2-, TLR3- or TLR4-dependent luciferase reporters were separately treated with LPS (1 μg/ml), PGN (1 μg/ml), and poly(I:C) (100 μg/ml) for 17 hours as shown in (a). TLR2, TLR3 and TLR4 were constructed in the HEK293 cell line. Cells were treated with individual ligands and different doses of CCM111 as shown in (b–d). After 17 hours, the luciferase activity was measured. The S.D. was performed by Student’s t-test compared to the individual ligand group. **p-value < 0.01 and ***p-value < 0.001. Results were obtained from three independent replicates.
