Figure 7
The effects of CCM111 fractions on NO production and the protein expressions of iNOS, COX-2 in murine RAW264.7 macrophages. (a) RAW264.7 cells were treated separately with different concentrations of CCM111 fractions for 24 hours. (b) The cells were treated with LPS (100 ng/ml) alone or in combination with different concentrations of CCM111 fractions. (c,d) The cells were treated with LPS (100 ng/ml) alone or in combination with different concentrations of fraction 4 for 24 hours. (e) The cells were treated with IL-6 (10ng/ml) alone or in combination with F4 for 0.5 hours. (a) After treatment, the survival of cells was detected by the Alamar Blue assay. (b,c) After incubation, the culture media were collected for nitrate assay analysis. (d,e) After treatment, the cell lysates were analyzed by immunoblotting. (d) The protein levels of iNOS and COX-2 were detected, and β-actin was used as the internal control. (e) The protein level of phospho-Tyk2 was detected, and GAPDH was used as the internal control. The full length blots images are presented in Supplementary Fig. 6. The S.D. was performed by Student’s t-test compared to the control or LPS only group. **p-value < 0.01 and ***p-value < 0.001. Results were obtained from three independent replicates.
