Figure 4

Determining the effect of secreted factors on failure of synthetic tissues made from young or pre-aged cells. (A) The timeline for conditioned media experiments. (B) The schematic of media transfer and (C) the live cell percentage change over time of synthetic tissues with 100 K pre-aged cells receiving 100 K young synthetic tissue conditioned or non-conditioned media. (D) The schematic of media transfer experiment of synthetic tissues made from young or pre-aged cells at 1 K density, receiving 100 K young or 100 K pre-aged synthetic tissue conditioned media. Synthetic tissues maintained in non-conditioned media are used as controls. (E) The live cell percentages of 1 K synthetic tissues receiving non-conditioned (control) or conditioned media. (F) The lifetime of synthetic tissues made from young or pre-aged cells at 1 K density receiving 100 K young or 100 K pre-aged synthetic tissue conditioned or non-conditioned media.