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Figure 1

From: Rapid production of human liver scaffolds for functional tissue engineering by high shear stress oscillation-decellularization

Figure 1

Decellularization of human liver cubes. (a) Macroscopic appearance and histological analysis after decellularization, confirming elimination of nuclear (blue; H&E) and cellular material (yellow; SR) and preservation of collagen (red; SR) and elastin (blue/black; EVG). (b) Macroscopic appearance and histological images of fresh human liver. (c) DNA quantification showing significant elimination of DNA in the decellularized cubes. (d) Comparison of the expression and distribution of several ECM proteins, namely collagen I, collagen III, collagen IV, fibronectin and Laminin, evaluated by immunohistochemistry showing consistency between decellularized cubes (top panel) and fresh samples (bottom panel). Data are expressed as mean ± s.d. ***p < 0.0001. Scale bars, 1 mm macroscopic images (a,b) or 200 μm top panel; a, b or 50 μm bottom panel; a, b or 100 μm (d). Biological replicates (n = 16) are performed for all samples.

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