Figure 3 | Scientific Reports

Figure 3

From: Hyperosmolarity-induced AQP5 upregulation promotes inflammation and cell death via JNK1/2 Activation in human corneal epithelial cells

Figure 3

p-JNK1/2 inhibitor suppressed hyperosmolarity-induced AQP5 protein expression. The immortalized HCEC cell viability was relatively unchanged in the 0.05%, 0.1%, 0.2%DMSO groups but decreased significantly in the 0.5% DMSO group, compared to the control group (P = 0.018) (A). The cell viability in 10 μM, 20 μM, and 40 μM SP60012 dissolved in 0.1%DMSO was not different from that of the control group, while in the 80 μM SP600125 group cell viability significantly decreased (P = 0.037) (B). Pre-treatment with 20 μM SP600125 decreased IL-1β, IL-6, IL-8, TNF-α, caspase-1, and AQP5 mRNA levels in 500 mOsm medium during 4 h (P ≤ 0.012) (C), and the protein expression levels of p-JNK1//2 (D) and AQP5 (E) also declined by 33.3% (P = 0.001) and 39.3% (P = 0.008) respectively after 24 h. The cropped gels are displayed and the full-length gels are provided in a Supplementary Information file. Experiments were repeated three times each in triplicate.

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